To provide insights on how to characterise multi-wythe masonry infrastructure, a pilot study has been started at Delft University of Technology in 2022 with the characterisation of a bridge’s pillar built in 1882 in Amsterdam (Bridge 41). Samples were extracted in the portion of masonry above water level and different samples through the thickness direction of the masonry wall were collected. Various mechanical tests, including tests on cores and on standard rectangular samples (e.g. couplets, prisms, triplets) were performed. In this dataset, the outcome of compressive tests on cores and prisms, splitting tests on cores, shear-compression tests on triplets, and bond wrench tests on couplets, are presented to characterise compressive properties of masonry as well as shear and bond properties of brick-mortar interfaces. The scope is to identify if it exists a variation of the mechanical properties throughout the thickness and to discuss the suitability of the core testing method for multi-wythe masonry in ...