Penalized splines are used in various types of regression analyses, including non-parametric quantile, robust and the usual mean regression. In this paper, we focus on the penalized spline estimator with general convex loss functions. By specifying the loss function, we can obtain the mean estimator, quantile estimator and robust estimator. We will first study the asymptotic properties of penalized splines. Specifically, we will show the asymptotic bias and variance as well as the asymptotic normality of the estimator. Next, we will discuss smoothing parameter selection for the minimization of the mean integrated squares error. The new smoothing parameter can be expressed uniquely using the asymptotic bias and variance of the penalized spline estimator. To validate the new smoothing parameter selection method, we will provide a simulation. The simulation results show that the consistency of the estimator with the proposed smoothing parameter selection method can be confirmed and that the proposed estimator has better behavior than the estimator with generalized approximate cross-validation. A real data example is also addressed.