The purpose of this study was to standardize the Vocational Preference Inventory for John Holland (VPI-1985) to the Jordanian environment, and examine the psychometric characteristics of the Jordanian version of the Inventory. The Jordanian was applied for (2252) Jordanian students (female N=1133) and (Male N=1119). Anticipated gender differences were found on the RIASEC scales, for the males who achieved the highest scores on the scales: Investigative, Realistic and Enterprising, while females got the highest score on the Investigate, Artistic and Social Scale. The results on the Personality Scales found that males achieved the highest score on the (Acquiescence, self-control and Infrequency) scales, while females achieved the highest scores on the (Acquiescence, Infrequency and self-control). An aspect of the results was the increase of the Investigative interest for females and that both males and females got the first rank on the Acquiescence scale. The results support the reliability and validity of Holland's theory and the vocational preference inventory for the Jordanian environment.