Objective: Investigate protein intake patterns over the day and their association with total protein intake in older adults. Design: Cross-sectional study utilising the dietary data collected through two non-consecutive, dietary record-assisted 24-h recalls. Days with low protein intake (n 290) were defined using the RDA (<0.8 g protein/kg adjusted BW/d). For each day, the amount and proportion of protein ingested at every hour of the day and during morning, mid-day and evening hours was calculated. Amounts and proportions were compared between low and high protein intake days and related to total protein intake and risk of low protein intake. Setting: Community. Participants: 739 Dutch community-dwelling adults ≥70 years. Results: The mean protein intake was 76.3 (sd 0.7) g/d. At each hour of the day, the amount of protein ingested was higher on days with a high protein intake than on days with a low protein intake and associated with a higher total protein intake. The proportion of protein ingested during morning hours was higher (22 v. 17 %, P < 0.0001) on days with a low protein intake, and a higher proportion of protein ingested during morning hours was associated with a lower total protein intake (P < 0.0001) and a higher odds of low protein intake (OR 1.04, 95 % CI 1.03, 1.06). For the proportion of protein intake during mid-day or evening hours, opposite but weaker associations were found. Conclusions: In this sample, timing of protein intake was associated with total protein intake. Additional studies need to clarify the importance of these findings to optimise protein intake.