Digestive aspartic proteases from sábalo (Prochilodus lineatus): Characterization and application for collagen extraction
Acid proteases from sábalo stomach mucosa were recovered using salting-out procedure. This single step produced an enzyme extract purified 1.8-fold over the crude extract with a recovery of 45.1% of its initial proteolytic activity. Sábalo proteases exhibited the highest activity at 45 °C-pH 2.0, showed pH stability between 2.0 and 5.0 and retained more than 70% of its activity after incubation at pH 7.0 for 2 h. Fish extract was unstable at temperatures greater than 45 °C. Its activity was inhibited by pepstatin A but not by PMSF, while EDTA and SDS showed partial inhibitory effects. Presence... Mehr ...
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Dokumenttyp: | Artikel |
Verlag/Hrsg.: |
Elsevier
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Schlagwörter: | ACETIC ACID (PUBCHEM CID: 176) / ACIDIC PROTEASE / BUTYL ALCOHOL (PUBCHEM CID: 263) / COLLAGEN / ETHYLENEDIAMINETETRAACETIC ACID (PUBCHEM CID: 6049) / GLYCINE (PUBCHEM CID: 750) / HYDROCHLORIC ACID (PUBCHEM CID: 313) / METHANOL (PUBCHEM CID: 887) / PHENYLMETHYLSULFONYL FLUORIDE (PUBCHEM CID: 4784) / SODIUM CHLORIDE (PUBCHEM CID: 5234) / SODIUM DODECYL SULFATE (PUBCHEM CID: 3423265) / SODIUM HYDROXIDE (PUBCHEM CID: 14798) / STOMACH / SÁBALO (PROCHILODUS LINEATUS) / https://purl.org/becyt/ford/1.4 / https://purl.org/becyt/ford/1 |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-29264989 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | http://hdl.handle.net/11336/96187 |