Impacts of rainforest degradation on the diets of the insectivorous bats of Sabah

Description: The work was carried out within Sabah, at the SAFE project, Danum Valley and Maliau basin. Bats were captured by deploying 6 harp traps per night, during field seasons taking place in 2015, 2016 and 2017. Bat guano samples were collected by placing individual bats into cloth bags, and then releasing them after 12 hours. Any guano in the bottom of the bag was then transferred into 95% ethanol and stored at -20. DNA was extracted from the faecal samples using a Qiagen Stool Mini kit, and then amplified using the ZBJ-ArtF1c ZBJ-ArtR2c primers, and sequencing the DNA on an Illumina Mi... Mehr ...

Verfasser: Hemprich-Bennett, David
Kemp, Victoria
Blackman, Joshua
Bernard, Henry
Lewis, Owen
Struebig, Matthew
Rossiter, Stephen
Clare, Elizabeth
Dokumenttyp: other
Erscheinungsdatum: 2019
Verlag/Hrsg.: Zenodo
Schlagwörter: Bats / DNA metabarcoding / Feeding ecology / Trophic interactions / Arthropods / Chiroptera / Borneo
Sprache: unknown
Permalink: https://search.fid-benelux.de/Record/base-29261549
Datenquelle: BASE; Originalkatalog
Powered By: BASE
Link(s) : https://doi.org/10.5281/zenodo.3247465

Description: The work was carried out within Sabah, at the SAFE project, Danum Valley and Maliau basin. Bats were captured by deploying 6 harp traps per night, during field seasons taking place in 2015, 2016 and 2017. Bat guano samples were collected by placing individual bats into cloth bags, and then releasing them after 12 hours. Any guano in the bottom of the bag was then transferred into 95% ethanol and stored at -20. DNA was extracted from the faecal samples using a Qiagen Stool Mini kit, and then amplified using the ZBJ-ArtF1c ZBJ-ArtR2c primers, and sequencing the DNA on an Illumina MiSeq. Project: This dataset was collected as part of the following SAFE research project: Impacts of rainforest degradation on the diets of the insectivorous bats of Sabah Funding: These data were collected as part of research funded by: NERC (Standard grant, NE/K016407/1) Royal Society (Standard grant, RG130793) Bat Conservation International (Standard grant) This dataset is released under the CC-BY 4.0 licence, requiring that you cite the dataset in any outputs, but has the additional condition that you acknowledge the contribution of these funders in any outputs. Permits: These data were collected under permit from the following authorities: Sabah Biodiversity Centre (Research licence JKM/MBS.1000-2/2 JLD.4 (46)) XML metadata: GEMINI compliant metadata for this dataset is available here Files: This dataset consists of 3 files: Bat_SAFE_data_metadata.xlsx, interaction_network.csv, sequences_95.fasta Bat_SAFE_data_metadata.xlsx This file contains dataset metadata and 1 data tables: data (described in worksheet Data) Description: measurements collected Number of fields: 21 Number of data rows: 3292 Fields: TrapName : The trap ID which the bats were captured in (Field type: ID) Lat : Latitude of trap (Field type: Latitude) Long : Longitude of trap (Field type: Longitude) Elevation : Elevation of trap (Field type: Numeric) Bat_no : Bat ID (Field type: ID) Date : Date of capture (Field type: Date) Faeces_no1 : Tube number used ...