The knowledge of mycorrhizal status of plants is very important both from ecological and conservation point of view, even more for endangered species and fragile ecosystems. Thus, this study aimed to evaluate and characterize the arbuscular mycorrhizal status of 20 Cuban endemic species present in the white sand savannas of Sabanalamar, Pinar del Río. Plants were sampled in October 2009 (end of the rainy season) and April 2010 (end of the following dry season). All species were arbuscular mycotrophic, and most of them showed high values of mycorrhizal colonization and visual density. No relationship was detected between seasonality and mycorrhizal association at the ecosystem level. Three plant groups were identified based on mycorrhizal association levels which suggests different strategies of mycorrhizal functioning. The Paris-type morphology was identified in all species. The Arum and Intermediate types were also present in 35 % and 15 % of the plant species, respectively, and always accompanied by the Paris-type. Root colonization by dark septate endophytes, fine endophyte and ectomycorrhiza were also observed in 19, seven and one species, respectively. This work contributes to the knowledge and characterization of the arbuscular mycorrhizal status of Cuban endemic species, as well as the reporting of other fungal root associations. Colonization by fine endophyte in Cuban plants is recorded for the first time. Citation: Rodríguez-Rodríguez, R.M., Sánchez, J.A. & Herrera, P.P. 2021. Mycotrophy of endemic plants of the white sand savannas of Pinar del Río, Cuba. Revista Jard. Bot. Nac. Univ. Habana 42: 163-173. Received: 21 October 2020. Accepted: 22 March 2021. Online: 21 July 2021. Editor: Milay Cabarroi. ; El conocimiento del estatus micorrízico de las plantas es muy importante tanto del punto de vista ecológico como conservacionista, aún más para especies amenazadas y ecosistemas frágiles. Por tanto, este estudio tuvo como objetivo evaluar y caracterizar el estatus micorrízico arbuscular de 20 especies ...