The access to healthcare in rural areas is difficult and telemedicine could be a promising solution to this problem if it is accepted by the population. The aim of this study was to assess different attitudes towards telemedicine, the level of confidence and acceptance of telemedicine among rural populations in Sabah. A questionnaire based on the Technology Acceptance Model (TAM) framework and other validated studies were developed to achieve the data for this study. The questionnaire was distributed to the residents of Penampang and Putatan in Sabah. The questions were scored using a five-level Likert scale and the level of confidence was rated from 0 to 100%. A high mean score of attitudes towards use and a moderate score of confidence was observed in the findings, indicating positive attitude towards telemedicine and reasonable level of confidence in using telemedicine services for their healthcare needs. Findings in this study revealed a high level of acceptance among the respondents, which was found to be generally consistent with previous telemedicine studies.