To evaluate the performance of organic and inorganic fertilizers for regeneration of ecosystem services in peat grasslands with biodiversity goals, we carried out a field experiment in the western peat district in the Netherlands. The fertilizers tested represent the current practice and potential alternatives for regenerative grassland management on drained peat. Experimental setup The field experiment (2013 – 2015) was conducted on a permanent grassland on peat soil (Terric Histosol; SOM 56 g 100 g −1 and pH KCl of 4.5 in 0-10 cm) at the experimental dairy farm at Zegveld (the Netherlands). In March 2013, a randomized block experiment (six blocks) was laid out with six fertilizer treatments and a control treatment (no fertilizer: “Contr”). The fertilizer used were: conventional dairy cattle slurry manure (“Slurry”), mature compost of kitchen and garden waste (“Comp”), dairy cattle farmyard manure (“FYM”), solid fraction of the cattle slurry manure (“SFrac”, obtained by pressurized filtration), inorganic N fertilizer (“IF”; calcium ammonium nitrate, 27% N) and a combination of inorganic N fertilizer and sawdust (“IF+SD”). Plot size was 4 × 10 m; for the Slurry treatment plots were 5.2 × 10 m. Slurry was applied by slit injection, the other fertilizers were applied by hand. Target application rate was 120 kg total N ha −1 yr −1 , divided in two applications per year (February/March and May). This is relatively low for conventional grasslands but usual for grasslands with biodiversity goals (Kleijn et al., 2004). The amount of C total applied in Comp was taken for the rate of sawdust to be applied. All plots were fertilized with 200 kg K 2 O ha −1 yr −1 (applications in March and May) (Commissie Bemesting Grasland en Voedergewassen, 2019). Fertilizer application quantities and organic matter and nutrient inputs are provided in Fertilizer_intput.csv (dataset). The grassland had an history of conventional management with mainly cutting, winter grazing with sheep and a normal fertilization regime with both slurry ...