The European Prospective Investigation into Cancer and Nutrition (EPIC), which hasbe en established in order to investigate the relations between nutrition and cancer, wasinitiated in 1990 and involves10 European countrieswith heterogeneous dietary patternsand differing cancer incidence rates. This manuscript presents the design, recruitment and baseline characteristics of the Prospect-EPIC cohort co-ordinated in Utrecht, The Netherlands. The cohort isba sed on volunteersrecrui ted among women participating in a regional breast cancer screening program. It comprises of 17,357 subjects aged 50â 69 yearsat enrolment from Utrecht and vicinity, who have consented to participate in the study and its follow-up. Each participant filled out a general questionnaire and a food frequency questionnaire. Participants were also physically examined and have donated a blood sample. Participation rate was 34.5%. Blood samples were donated by most participants(97.5%) and detailed informed consentswer e obtained from 87.4% of participants. Mean age at enrolment was 57 years. Anthropometric, lifestyle and morbidity characteristics of the cohort population did not differ largely from those of similar study populations in The Netherlands. Based on the Prospect-EPIC population, we intend to conduct prospective total cohort, nested caseâ control or caseâ cohort studies, in order to investigate relations between consumption of certain food groups or nutrients and chronic diseases, including hormone dependant cancers such as breast, colon, endometrial and ovary cancers.