Influence of volume of sample processed on detection of Chlamydia trachomatis in urogenital samples by PCR

In the present study, it was demonstrated that the sensitivity of the PCR for the detection of Chlamydia trachomatis is influenced by the volume of the clinical sample which is processed in the PCR. An adequate sensitivity for PCR was established by processing at least 4%, i.e., 80 microliters, of the clinical sample volume per PCR. By using this preparation procedure, 1,110 clinical samples were evaluated by PCR and by cell culture, and results were compared. After discordant analysis, cell culture resulted in a sensitivity of 79.1% and PCR resulted in a sensitivity of 92.7%. Furthermore, it... Mehr ...

Verfasser: Goessens, W.H.F. (Wil)
Kluytmans, J.A.J.W. (Jan)
Toom, N. den
Rijsoort-Vos, T.H. van
Stolz, E. (Ernst)
Verbrugh, H.A. (Henri)
Quint, W.G.V. (Wim)
Niesters, H.G.M. (Bert)
Dokumenttyp: Artikel
Erscheinungsdatum: 1995
Schlagwörter: Cervix Uteri/microbiology / Chlamydia Infections/*diagnosis/epidemiology / Chlamydia trachomatis/genetics/*isolation & purification / False Negative Reactions / False Positive Reactions / Female / Humans / Male / Netherlands/epidemiology / Polymerase Chain Reaction/*methods/standards / Prevalence / Prospective Studies / Sensitivity and Specificity / Urethra/microbiology
Sprache: Englisch
Permalink: https://search.fid-benelux.de/Record/base-29199595
Datenquelle: BASE; Originalkatalog
Powered By: BASE
Link(s) : http://repub.eur.nl/pub/8549

In the present study, it was demonstrated that the sensitivity of the PCR for the detection of Chlamydia trachomatis is influenced by the volume of the clinical sample which is processed in the PCR. An adequate sensitivity for PCR was established by processing at least 4%, i.e., 80 microliters, of the clinical sample volume per PCR. By using this preparation procedure, 1,110 clinical samples were evaluated by PCR and by cell culture, and results were compared. After discordant analysis, cell culture resulted in a sensitivity of 79.1% and PCR resulted in a sensitivity of 92.7%. Furthermore, it was shown that treatmen