Mitochondrial DNA damage analysis in bronchoalveolar lavage cells of preterm infants
In mechanically ventilated preterm infants, the combination of immaturity, volutrauma, oxidative stress, and inflammatory processes can lead to chronic lung injury. Mitochondrial DNA (mtDNA) is more susceptible to oxidative damage than nuclear DNA. We aimed to investigate the level of mtDNA damage (deletions, mutations and changes in copy number) in bronchoalveolar lavage (BAL) cells from 10 preterm infants (27-30 weeks). A first BAL (BAL1) was done within 24 h of endotracheal intubation and BAL2 was performed 30-103 h thereafter. Deletions were analyzed by long range PCR, point mutations by h... Mehr ...
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Dokumenttyp: | Artikel |
Erscheinungsdatum: | 2010 |
Reihe/Periodikum: | Zoer , B , Been , J V , Jongen , E , Debeer , A , Hendrickx , A , Smeets , H J M , Zimmermann , L J & Villamor , E 2010 , ' Mitochondrial DNA damage analysis in bronchoalveolar lavage cells of preterm infants ' , Frontiers in Bioscience, Elite edition , vol. 1 , no. 2 , pp. 361-368 . https://doi.org/10.2741/E96 |
Schlagwörter: | Bronchoalveolar Lavage Fluid / DNA Copy Number Variations / DNA Damage / DNA Mutational Analysis / DNA Primers / DNA / Mitochondrial / Humans / Infant / Newborn / Netherlands / Premature Birth / Respiration / Artificial / Journal Article / Research Support / Non-U.S. Gov't |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-29187166 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://cris.maastrichtuniversity.nl/en/publications/8139b1a0-b1b4-45e9-a355-ec8696536f84 |