Two-tiered SARS-CoV-2 seroconversion screening in the Netherlands and stability of nucleocapsid, spike protein domain 1 and neutralizing antibodies ...

Serological testing in the COVID-19 pandemic is mainly implemented to gain sero-epidemiological data, but can also retrospectively inform about suspected SARS-CoV-2 infection. We verified and applied a two-tiered testing strategy combining a SARS-CoV-2 receptor-binding domain (RBD)-specific lateral flow assay (LFA) with a nucleocapsid protein (NCP) IgG ELISA to assess seroconversion in n = 7241 individuals. The majority had experienced symptoms consistent with COVID-19, but had no access to RT-PCR testing. Longitudinal follow-up in n = 97 LFA + individuals was performed up to 20 weeks after in... Mehr ...

Verfasser: Garritsen, Anja
Scholzen, Anja
Van Den Nieuwenhof, Daan W. A.
Smits, Anke P. F.
E. Suzan Datema
Van Galen, Luc S.
Kouwijzer, Milou L. C. E
Dokumenttyp: dataset
Erscheinungsdatum: 2021
Verlag/Hrsg.: Taylor & Francis
Schlagwörter: Medicine / Neuroscience / Biotechnology / Immunology / FOS: Clinical medicine / 80699 Information Systems not elsewhere classified / FOS: Computer and information sciences / 19999 Mathematical Sciences not elsewhere classified / FOS: Mathematics / Cancer / 110309 Infectious Diseases / FOS: Health sciences / 60506 Virology / FOS: Biological sciences / Computational Biology
Sprache: unknown
Permalink: https://search.fid-benelux.de/Record/base-29165756
Datenquelle: BASE; Originalkatalog
Powered By: BASE
Link(s) : https://dx.doi.org/10.6084/m9.figshare.14179954.v1

Serological testing in the COVID-19 pandemic is mainly implemented to gain sero-epidemiological data, but can also retrospectively inform about suspected SARS-CoV-2 infection. We verified and applied a two-tiered testing strategy combining a SARS-CoV-2 receptor-binding domain (RBD)-specific lateral flow assay (LFA) with a nucleocapsid protein (NCP) IgG ELISA to assess seroconversion in n = 7241 individuals. The majority had experienced symptoms consistent with COVID-19, but had no access to RT-PCR testing. Longitudinal follow-up in n = 97 LFA + individuals was performed up to 20 weeks after initial infection using NCP and spike protein S1 domain (S1) IgG ELISAs and a surrogate virus neutralization test (sVNT). Individuals reporting symptoms from January 2020 onwards showed seroconversion, as did a considerable proportion of asymptomatic individuals. Seroconversion for symptomatic and asymptomatic individuals was higher in an area with a known infection cluster compared to a low incidence area. Overall, 94% ...