PA content J. vulgaris ...

PA analysis of leaf and root samples was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) following the procedure outlined in Kostenko et al. (2013). In brief, 5 mg of freeze-dried ground plant material was extracted with 0.5 ml 2% formic acid solution containing heliotrine (1 µg•ml-1) as internal standard. After centrifugation and filtration, 25 µl of the extracted filtrate was diluted with 975 µl of 10 mM ammonium hydroxide solution and 10 µl was injected in a Waters Acquity ultra-performance chromatographic system coupled to a Waters Quattro Premier tandem mass sp... Mehr ...