Cryptosporidium proventriculi in Captive Cockatiels (Nymphicus hollandicus)

Cockatiels (Nymphicus hollandicus) are among the most commonly sold psittacines pets. The aim of this study was to evaluate the occurrence of Cryptosporidium spp. in domestic N. hollandicus and identify risk factors for this infection. We collected fecal samples from 100 domestic cockatiels in the city of Araçatuba, São Paulo, Brazil. Feces from birds of both genders and older than two months were collected. Owners were asked to complete a questionnaire to identify how they handle and care for their birds. Based on nested PCR targeting the 18S rRNA gene, the prevalence of Cryptosporidium spp.... Mehr ...

Verfasser: Mariele Fernanda da Cruz Panegossi
Giovanni Widmer
Walter Bertequini Nagata
Bruno César Miranda Oliveira
Elis Domingos Ferrari
Jancarlo Ferreira Gomes
Marcelo Vasconcelos Meireles
Alex Akira Nakamura
Thaís Rabelo do Santos-Doni
Luiz da Silveira Neto
Katia Denise Saraiva Bresciani
Dokumenttyp: Text
Erscheinungsdatum: 2023
Verlag/Hrsg.: Multidisciplinary Digital Publishing Institute
Schlagwörter: cryptosporidiosis / birds / epidemiology / molecular characterization / 18S rRNA gene / prevalence / risk factors
Sprache: Englisch
Permalink: https://search.fid-benelux.de/Record/base-29076087
Datenquelle: BASE; Originalkatalog
Powered By: BASE
Link(s) : https://doi.org/10.3390/pathogens12050710

Cockatiels (Nymphicus hollandicus) are among the most commonly sold psittacines pets. The aim of this study was to evaluate the occurrence of Cryptosporidium spp. in domestic N. hollandicus and identify risk factors for this infection. We collected fecal samples from 100 domestic cockatiels in the city of Araçatuba, São Paulo, Brazil. Feces from birds of both genders and older than two months were collected. Owners were asked to complete a questionnaire to identify how they handle and care for their birds. Based on nested PCR targeting the 18S rRNA gene, the prevalence of Cryptosporidium spp. in the cockatiels sampled was 9.00%, 6.00% based on Malachite green staining, 5.00% based on modified Kinyoun straining, and 7.00% when the Malachite green was combined with Kinyoun. Applying multivariate logistic regression to test the association between Cryptosporidium proventriculi positivity and potential predictors showed that gastrointestinal alterations was a significant predictor (p < 0.01). Amplicons from five samples were sequenced successfully and showed 100% similarity with C. proventriculi. In summary, this study demonstrates the occurrence of C. proventriculi in captive cockatiels.