Background: The Child Sexual Behavior Inventory (CSBI) is one of the few instruments available to assess sexual behavior in children. Because of cross-cultural differences in the definition of normal and atypical sexual behaviors, the CSBI is not generalizable to other countries. Objective: The current study examined psychometric properties of the Dutch version of the CSBI. Participants and setting: The study represents 3206 children from a normative sample, and 932 children from clinical samples. Methods: Parents ratings on the CSBI, and questionnaires for psychosocial problems were obtained to assess psychometric properties. Results: Results showed that Dutch parents interpret more CSBI items as developmentally appropriate than USA parents. Reliability coefficients indicated internal consistency (α = .39–.82), test-retest (r = .86, p < .000) and interrater reliability (Cohen's d =0.47, p >.05). Positive correlations between the CSBI total scale and the subscales demonstrated content validity (r = .32–.96, p < .01). Differences between normative and clinical samples on the CSBI total (ηp 2 = .01–.65, p < .000), DRSB (ηp 2 = .00–.03, p = .00–.38) and SASI scales (ηp 2 =.00–.18, p < .01) indicated construct validity. Within the clinical samples, highest associations were found between the CSBI score and convergent measures (r = .64, p < .000). Lowest associations were found between the CSBI scores and divergent measures r = .14–.54, p = n.s.−<.000. Conclusions: Cross-cultural differences in the perception of developmental appropriate sexual behavior underscore the importance of country-specific normative data. Good psychometric properties of the Dutch version of the CSBI were supported by this study.