During a routine survey conducted in July 2021, several dead and dying Ulmus macrocarpa trees were observed in an urban forest located in the Gyeonggi Province of South Korea. The trees had symptoms of wilt with yellowing and browning of leaves, and, in most cases, the trunks of dying trees were infested by bark beetles. Isolations were made from small pieces of wood taken from dying trees, and beetles were collected from the infested stems. Fungal isolates and the beetles were identified using DNA sequence-based phylogenies and morphology, respectively. The results revealed that the fungus was Ophiostoma novo-ulmi, the causal agent of Dutch elm disease, and the associated bark beetle was Scolytus jacobsoni. This study provides the first record of Dutch elm disease in South Korea and suggests that a nationwide survey for the disease should be undertaken. ; This work was supported by a project, Investigation and characterization on occurrence of emerging forest insect pests and fungal diseases (Project No. FE0702-2021-01), from the National Institute of Forest Science, Republic of Korea. ; https://www.mdpi.com/journal/forests ; Biochemistry ; Forestry and Agricultural Biotechnology Institute (FABI) ; Genetics ; Microbiology and Plant Pathology