Application of shotgun metagenomics sequencing and targeted sequence capture to detect circulating porcine viruses in the Dutch–German border region
Porcine viruses have been emerging in recent decades, threatening animal and human health, as well as economic stability for pig farmers worldwide. Next-generation sequencing (NGS) can detect and characterize known and unknown viruses but has limited sensitivity when an unbiased approach, such as shotgun metagenomics sequencing, is used. To increase the sensitivity of NGS for the detection of viruses, we applied and evaluated a broad viral targeted sequence capture (TSC) panel and compared it to an unbiased shotgun metagenomic approach. A cohort of 36 pooled porcine nasal swab and blood serum... Mehr ...
Verfasser: |
Schuele, Leonard
Lizarazo-Forero, Erley
Strutzberg-Minder, Katrin
Schütze, Sabine
Löbert, Sandra
Lambrecht, Claudia
Harlizius, Jürgen
Friedrich, Alex W.
Peter, Silke
Rossen, John W.A.
Couto, Natacha |
Dokumenttyp: |
Artikel |
Erscheinungsdatum: |
2022 |
Reihe/Periodikum: |
Schuele , L , Lizarazo-Forero , E , Strutzberg-Minder , K , Schütze , S , Löbert , S , Lambrecht , C , Harlizius , J , Friedrich , A W , Peter , S , Rossen , J W A & Couto , N 2022 , ' Application of shotgun metagenomics sequencing and targeted sequence capture to detect circulating porcine viruses in the Dutch–German border region ' , Transboundary and Emerging Diseases , vol. 69 , no. 4 , pp. 2306-2319 . https://doi.org/10.1111/tbed.14249 |
Schlagwörter: |
influenza A virus
/ one health
/ porcine virome
/ shotgun metagenomics sequencing
/ surveillance
/ targeted sequence capture
/ /dk/atira/pure/subjectarea/asjc/2400
/ name=Immunology and Microbiology(all)
/ /dk/atira/pure/subjectarea/asjc/3400
/ name=veterinary(all)
/ /dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
/ name=SDG 3 - Good Health and Well-being |
Sprache: |
Englisch |
Permalink: |
https://search.fid-benelux.de/Record/base-29031510 |
Datenquelle: |
BASE;
Originalkatalog |
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Link(s) :
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https://researchportal.bath.ac.uk/en/publications/b7618bb5-bc49-495a-bdd8-09bb73243524 |