Daycare centers (DCCs) are where infants and toddlers (0–4 years old) spend the most time besides their homes. Given their higher susceptibility to the effects of air pollutants, as compared to older children and adults, indoor air quality (IAQ) is regarded as an essential parameter to monitor in DCCs. Recent advances in IAQ monitoring technologies have enabled the deployment of low-cost air quality monitors (LCMs) and single sensors (LCSs) to continuously monitor various indoor environments, and their performance testing should also be performed in the intended indoor applications. To our knowledge, there is no study evaluating the application of LCMs/LCSs in DCCs scenarios yet.Therefore, this study is aimed to assess the response of five types of LCMs (previously not tested) and five LCSs to typical DCCs emission activities in detecting multiple IAQ parameters, i.e., particulate matter, carbon dioxide, total volatile organic compounds, temperature, and relative humidity. These LCMs/LCSs were compared to outcomes from research-grade instruments (RGIs). All the experiments were performed in a climate chamber, where three kinds of typical activities (background; arts-and-crafts; cleaning; [in a total of 32 events]) were simulated by recruited subjects at two typical indoor climatic conditions (cool and dry [20 ± 1 °C & 40 ± 10%], warm and humid [26 ± 1 °C & 70 ± 5%]).Results showed that tested LCMs had the ability to capture DCCs activities by simultaneously monitoring multiple IAQ parameters, and LCMs/LCSs revealed a strong correlation with RGIs in most events (R2 values from 0.7 to 1), but, for some events, the magnitude of responses varied widely. Sensirion SCD41, an emerging CO2 sensor built on the photoacoustic sensing principle, had a more accurate performance than all tested NDIR-based CO2 sensors/monitors. In general, the study implies that the selection of LCMs/LCSs for a specific application of interest should be based on emission characteristics and space conditions.