Geochemical tracers incorporated into the skeleton of reef-building corals are ideal proxies for reconstructing environmental parameters of ambient seawater such as temperature and salinity at subseasonal resolution. However, validation concerns of these environmental proxies due to the complex skeleton of some tropical Atlantic corals have hindered such coral-based environmental reconstructions in this region compared to the tropical Pacific. In order to identify complications associated with the complex skeletal architecture of the massive brain coral Diploria strigosa, we performed microsampling experiments along and across individual skeletal elements. We demonstrate that the mesoscale heterogeneity of Sr/Ca, d18O and d13C is a systematic feature of D. strigosa and is linked to different vital effects between skeletal elements. The thecal wall is significantly depleted in Sr, 18O and 13C compared to the adjacent septa and columella and differences between apparent temperature signatures of several ... : Supplement to: Giry, Cyril; Felis, Thomas; Kölling, Martin; Scheffers, Sander R (2010): Geochemistry and skeletal structure of Diploria strigosa, implications for coral-based climate reconstruction. Palaeogeography, Palaeoclimatology, Palaeoecology, 298, 378-387 ...