The presence and persistence of natural organic matter (NOM) in drinking water treatment plants (WTPs) requires a robust and rapid monitoring method. Measurement and monitoring of NOM fractions using current technology is time-consuming and expensive. This study uses fluorescence measurements in combination with Parallel Factor (ParaFac) analysis to characterize NOM fractions. This was achieved through: (1) determining the origin and composition of NOM fractions using fluorescence index ( FI ), humification index, biological index, and freshness index, and (2) using multivariate analysis to reveal key parameters and hidden NOM fraction characteristics influenced by seasonal changes and environmental activities. The ParaFac model revealed that the NOM fractions for Belgium plants are mainly hydrophobic acids, aromatic proteins, biological activity, humic acid-like, and fulvic acid-like moieties. The NOM fractions in South African plants were mainly aromatic protein, humic acid-like, fulvic acid-like, ...