Background: Seasonal influenza-like illness (ILI) affects millions of people yearly. Severe acute respira-tory infections (SARI), mainly influenza, are a lead-ing cause of hospitalisation and mortality. Increasing evidence indicates that non-influenza respiratory viruses (NIRV) also contribute to the burden of SARI. In Belgium, SARI surveillance by a network of sentinel hospitals has been ongoing since 2011. Aim: We report the results of using in-house multiplex qPCR for the detection of a flexible panel of viruses in respiratory ILI and SARI samples and the estimated incidence rates of SARI associated with each virus. Methods: We defined ILI as an illness with onset of fever and cough or dysp-noea. SARI was defined as an illness requiring hospi-talisation with onset of fever and cough or dyspnoea within the previous 10 days. Samples were collected in four winter seasons and tested by multiplex qPCR for influenza virus and NIRV. Using catchment population estimates, we calculated incidence rates of SARI asso-ciated with each virus. Results: One third of the SARI cases were positive for NIRV, reaching 49.4% among children younger than 15 years. In children younger than 5 years, incidence rates of NIRV-associated SARI were twice that of influenza (103.5 vs 57.6/100,000 person-months); co-infections with several NIRV, respiratory syncytial viruses, human metapneumovi-ruses and picornaviruses contributed most (33.1, 13.6, 15.8 and 18.2/100,000 person-months, respectively). Conclusion: Early testing for NIRV could be beneficial to clinical management of SARI patients, especially in children younger than 5 years, for whom the burden of NIRV-associated disease exceeds that of influenza. ; Belgian Federal Public Service 'Health, Food Chain Safety, and Environment'; Belgian National Insurance Health Care (INAMI/RIZIV); Regional Health Authorities of Flanders (Agentschap Zorg en Gezondheid, AZG); Brussels (Commission com-munautaire commune de Bruxelles-Capitale, COCOM); Wallonia (Agence pour une Vie de Qualite, AVIQ)