Antimicrobial stewardship (AMS) is an essential step in limiting the selection of antimicrobial resistance (AMR). A questionnaire was filled-out by 122 last-year students of Veterinary Medicine of Ghent University (UGhent) and Liege University (ULiege) to assess their perception of preparedness, the education quality, and their satisfaction regarding the education of AMS and AMR. UGhent students felt better prepared to clinically use antimicrobial agents (3.23 versus 2.98, p = 0.03). There were no statistically significant differences in the other two fields of perception of preparedness, i. e. pharmacology and AMR knowledge. The overall result of the knowledge score was 4.68 (sd 1.26) out of 8, and there was no statistically significant difference between the results of the two faculties. Less than 50% of the students were satisfied with their education in AMS and AMR. Most participants acknowledged the need for extra education in rational antibiotic use. UGhent students were more familiar with guidelines for antimicrobial use than ULiege students. The results of this study can help improve the quality of veterinary education in AMS and AMR in Belgium.