Antimicrobial resistance (AMR) poses a substantial global health threatwith profound economic implications. Acknowledging the imperative for a One Health (OH) strategyto combat this menace, Belgium introduced an annual national OH report, known as the “BELMAPreport,” encompassing antimicrobial use (AMU) and AMR, with the first edition completed in 2021.The integration of innovations for the healthcare system demands a meticulously planned process. Methods. We introduced a three-step stakeholder analysis (SA) as a prospective framework fornavigating this new report process, fostering complementary collaboration, pinpointing obstacles,suggesting approaches to overcome them, and facilitating national policy development. The SAunfolds in three steps: stakeholders identify and list their relevant activities, assess their positions re-garding the BELMAP report, and complete “actor mapping” of national AMR and AMU stakeholders. Results. Stakeholder identification reveals a fragmented landscape of AMR and AMU ...