It is becoming increasingly difficult to combine nature conservation by farmers with intensive and large-scale farming. The Dutch government recently adopted the new policy concept of ‘nature-inclusive’ farming, which aims at promoting more sustainable agricultural practices that minimizes negative ecological impacts, maximizes positive ones and at the same time benefits from natural processes. A transformation towards ‘nature-inclusive’ farming faces three key governance challenges that are elaborated upon in this paper. First, agri-environment schemes and other conservation arrangements need to become more effective. At the same time, nature conservation should be mainstreamed in agricultural policies and in agri-food chains. Second, we need shared meanings about nature-inclusive farming. Third, other forms of knowledge production for nature-inclusive farming are required that focus more on farmers’ knowledge needs.