Active coalitions of regional stakeholders are at the heart of contemporary regional economic development policies, such as Smart Specialisation or Constructing Regional Advantage. These coalitions consist of actors from various organizations such as regional authorities, companies and higher education institutions that come together to achieve common agendas and advance their region. Accordingly, the numerous stakeholders are expected to work together seamlessly, build and implement strategies and thereby deliver regional development. However, by assuming that strategy formulation and implementation is straightforward, the challenges that lie within partnerships and the tensions that may arise between stakeholders can become neglected. Therefore, it is vital to understand tensions that drive towards situations in which strategy-building is not successful and ‘black holes’ of strategy-building emerge. By identifying the tensions between regional partners in the Twente region of the Netherlands, the aim is to understand how such stakeholder tensions affect regional development. It is assumed that by easing or resolving these tensions, stakeholder partnerships can contribute to the successful advancement of their region. The data for this qualitative case study are drawn from both research interviews and secondary sources.