Helicobacter pullorum in Chickens, Belgium

A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic diversity and relatedness. Sixteen isolates from cecal samples from 2 different flocks were obtained. AFLP analysis showed that these isolates and 4 additional isolates from a different flock clustered according to their origin, which indicates that H. pullorum colonization may occur with a single... Mehr ...

Verfasser: Liesbeth M. Ceelen
Annemie Decostere
Kathleen Van den Bulck
Stephen L.W. On
Margo Baele
Richard Ducatelle
Freddy Haesebrouck
Dokumenttyp: Artikel
Erscheinungsdatum: 2006
Reihe/Periodikum: Emerging Infectious Diseases, Vol 12, Iss 2, Pp 263-267 (2006)
Verlag/Hrsg.: Centers for Disease Control and Prevention
Schlagwörter: Helicobacter pullorum / poultry / isolation / PCR / amplified fragment length polymorphism / research / Medicine / R / Infectious and parasitic diseases / RC109-216
Sprache: Englisch
Permalink: https://search.fid-benelux.de/Record/base-28563566
Datenquelle: BASE; Originalkatalog
Powered By: BASE
Link(s) : https://doi.org/10.3201/eid1202.050847

A total of 110 broilers from 11 flocks were tested for Helicobacter pullorum by polymerase chain reaction; positive samples were reexamined with a conventional isolation method. H. pullorum isolates were examined by amplified fragment length polymorphism (AFLP) fingerprinting for interstrain genetic diversity and relatedness. Sixteen isolates from cecal samples from 2 different flocks were obtained. AFLP analysis showed that these isolates and 4 additional isolates from a different flock clustered according to their origin, which indicates that H. pullorum colonization may occur with a single strain that disseminates throughout the flock. Strains isolated from different hosts or geographic sources displayed a distinctive pattern. H. pullorum is present in approximately one third of live chickens in Belgium and may represent a risk to human health.