Differential contributions of Saccharomyces cerevisiae and Brettanomyces claussenii to a Belgian strong beer
Parallel 49 Brewing Company has become an award-winning microbrewery at the heart of British Columbia’s growing beer culture. Wild Ride, one of their most successful beers, is brewed with a co-culture of Saccharomyces cerevisiae and Brettanomyces claussenii (synonym B. anomalus). While the use of co-cultures in brewing represents a profitable niche market, Parallel 49 has ceased production of Wild Ride as these fermentations are technically challenging and difficult to reproduce. In order to support the development of alternative production methods, Parallel 49 needs to gain an understanding o... Mehr ...
Dokumenttyp: | Text |
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Erscheinungsdatum: | 2019 |
Verlag/Hrsg.: |
Thompson Rivers University
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Schlagwörter: | Saccharomyces cerevisiae / Brettanomyces / Brewing -- Microbiology / Beer |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-28489085 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://arcabc.ca/islandora/object/tru%3A5110 |
Parallel 49 Brewing Company has become an award-winning microbrewery at the heart of British Columbia’s growing beer culture. Wild Ride, one of their most successful beers, is brewed with a co-culture of Saccharomyces cerevisiae and Brettanomyces claussenii (synonym B. anomalus). While the use of co-cultures in brewing represents a profitable niche market, Parallel 49 has ceased production of Wild Ride as these fermentations are technically challenging and difficult to reproduce. In order to support the development of alternative production methods, Parallel 49 needs to gain an understanding of the genomic profiles of the two yeast strains, profile yeast metabolites in relation to gene expression, and understand the genetic and metabolic interactions during co-culture fermentation. I hypothesize that, during co-culture fermentation, the “omic” profiles of the two yeast strains will be altered, and that there will be detectable interactions between the two strains. In order to test this hypothesis, a S. cerevisiae mono-culture brew, a B. claussenii mono-culture brew, and a Wild Ride co-culture brew were carried out. Fermentations proceeded for twenty-two days according to Parallel 49’s recipe, and specific gravity, dissolved oxygen and pH were monitored. Daily samples were taken for metabolite analysis via heated headspace gas chromatography coupled to a flame ionization detector and a mass spectrometer, transcriptomic analysis via RNA-seq on an Ion S5 System, and proteomic analysis via Waters Synapt G2 high definition mass spectrometry (Q-TOF MS) coupled to a nanoAcquity ultra performance liquid chromatography system. The genomes of both yeast cultures were sequenced on an Ion S5 System. To date, the day 7 transcriptomic profile of the Wild Ride co-culture has been sequenced, producing 9,734,188 Q20 reads and >= 1,620,889,603 Q20 bases. The Wild Ride reads were mapped to the Saccharomyces cerevisiae S288C reference genome at 17.65%, with 76.93% of the reads mapping to rRNA regions located on chromosome XII. ...