We performed a forest inventory in a fragment of stricto sensu Cerrado to evaluate alpha and beta temporal diversity among adult and natural regeneration (NR) strata. Fifteen plots of 1,000 m2 (20 × 50 m) were set to sample the adult stratum, and each plot divided in sub-plots of 100 m2 (2 × 50 m) to sample NR. We registered the qualitative (species) and quantitative (height and diameter) floristic composition. Natural Regeneration stratum was divided into three diameter classes (I, II and III). Moreover, species collected on non-oriented paths were registered. We estimated alpha diversity through Shannon index (H'), Pielou index (J') and "one-step" jackknife estimator. Sørensen (Sø- qualitative) and Czekanowski (SC - quantitative) indexes were used to evaluate beta diversity. In total, 101 species distributed in 71 genera and 36 families were recorded. For the adult and NR strata, H' values were 3.06 and 3.14 nats per individual, and J' 0.75 and 0.71, respectively. Both strata had qualitative similarity. Natural regeneration showed no similarity with adult stratum by Czekanowski index, although this distance tends to decline with the progressive size of each class. The temporal beta diversity is low between these strata; and there is a trend of increasing similarity with the maturity of NR. ; Un inventario forestal se realizó en un fragmento de Cerrado stricto sensu donde se evaluaron los índices de diversidad temporal alfa y beta entre los estratos adulto y regeneración natural (RN). Se establecieron quince parcelas de 1.000 m2 (20 × 50 m) para el estrato adulto de la muestra, y para el estrato de RN se establecieron sub-parcelas de 100 m2 (2 × 50 m). Se registraron parámetros cualitativos (especies) y cuantitativos (altura y diámetro) para evaluar la composición florística. El estrato de RN se dividió en tres clases diamétricas (I, II y III). Por otra parte, se realizó el registro de las especies encontradas en trayectos no-orientados. La diversidad alfa se estimó por Índice de Shannon (Η'), Pielou (J') y el ...