Kinetics and significance of the activity of the Sabath and Abrahams's {beta}-lactamase of Pseudomonas aeruginosaagainst cefotaxime and cefsuloding

All Pseudomonas aeruginosa strains produce Sabath and Abrahams' (SA) enzyme, as inducible β-lactamase. Hydrolysis of cefotaxime by this enzyme, although slow in terms of V max was efficient when the parameter V max /Km (physiological efficiency (Pollock, 1965)) was considered. Hydrolysis of cefsulo–din was not detectable in assays used and enzyme binding (Ki) was poor, ensuring a very low physiological efficiency. Physiological efficiency represents a measure of enzyme function under low substrate (antibiotic) conditions, as apply in the periplasm; consequently SA enzyme might protect the cell... Mehr ...