Abstract We investigate the stratigraphy of Neogene and Quaternary intervals of the Schelphoek borehole (Schouwen, Zeeland, the Netherlands). The Breda Formation (Miocene-Zanclean) contains three sequences separated by hiatuses. The Oosterhout Formation (Zanclean-Piacenzian) contains at least two sequences. This formation is overlain by seven sequences of the Gelasian Maassluis Formation that almost certainly represent glacial cycles. The three lowermost sequences are provisionally assigned to the Praetiglian (MIS 96, MIS 98 and MIS 100). A large hiatus exists between the top of the Maassluis Formation and the base of the late Middle to Late Quaternary succession. Due to extensive in situ reworking of older strata (including fossils) at the base of several of the formations, their exact boundaries are difficult to establish. The Neogene succession in the Schelphoek borehole is compared to the stratigraphic successions in the Antwerp area to the south and the Dutch coastal area and continental platform to the north. Finally, the stratigraphic context of the Gelasian (‘Tiglian’) mammal fauna dredged from the bottom of a major tidal channel in the adjacent Oosterschelde is assessed by comparison with the Schelphoek borehole.