{beta}-Lactamases among extended-spectrum {beta}-lactamase (ESBL)-resistant Salmonella from poultry, poultry products and human patients in The Netherlands

Objectives : The purpose of this work was to study the genetic determinants responsible for extended-spectrum β-lactamase (ESBL) resistance of Salmonella isolated from Dutch poultry, poultry meat and hospitalized humans. Methods : Thirty-four ESBL-resistant Salmonella isolates from The Netherlands were tested towards 21 antimicrobial agents. PCR and sequencing were used to determine the underlying genetic determinants responsible for the ESBL phenotypes. The transferability of the ESBL phenotypes was tested by conjugation to a susceptible Salmonella enterica serovar Dublin and plasmid purifica... Mehr ...