Horses in all equestrian disciplines are suffering from tendon injuries followed by several months of rehabilitation. Additionally, the scar tissue formed after injury is of inferior quality, resulting in high re-injury rates. Conventional treatments include rest, controlled exercise and anti-inflammatory drugs administration. In order to improve tissue regeneration and prevent reinjury, biological treatments, such as mesenchymal stem cells (MSCs) are gaining popularity. In this survey, the use of MSCs as treatment for tendon injuries by Flemish equine veterinary practitioners was evaluated. Although the respondents were in general unsatisfied with conventional therapy, it remains the preferred treatment. Approximately half of the survey population (47%) have already used MSCs therapy to treat tendon injuries. The other participants reported the high costs and complexity as major limitations. According to the respondents, the availability of strong scientific evidence of the mechanisms of action, accompanied by clear practical guidelines, could help to increase the practical application of MSC therapy.