In the present study, the first outbreak of a penguinpox virus (PPV) in Humboldt penguins ( Spheniscus humboldti ) and four outbreaks of fowlpox virus (FPV) in layer chickens are reported. Clinically, cutaneous wart-like growths were observed around the eyes in four juvenile Humboldt penguins and cutaneous nodular lesions in the comb, wattles, around the eyes and other unfeathered skin parts of layer chickens. Histopathology (FPV and PPV), electron microscopy (PPV), virus isolation (FPV) and PCR amplification (FPV and PPV) confirmed that both isolates were avipoxviruses (APVs). According to the phylogenetic analysis of the partial P4b core protein gene, the Belgian Humboldt PPV clustered with sequences of free-range (domestic and synanthrope bird species) and wild bird species of the United States and Europe (99 - 100% homology), and all four Belgian FPV isolates clustered with FPV isolates of chickens, turkeys, canary and FPV attenuated live vaccines from all over the world (100% homology).