Optimization and application of a multiplex digital PCR assay for the detection of SARS-CoV-2 variants of concern in Belgian influent wastewater
Since the beginning of the COVID-19 pandemic, the wastewater-based epidemiology (WBE) of SARS-CoV-2 has been used as a complementary indicator to follow up on the trends in the COVID-19 spread in Belgium and in many other countries. To further develop the use of WBE, a multiplex digital polymerase chain reaction (dPCR) assay was optimized, validated and applied for the measurement of emerging SARS-CoV-2 variants of concern (VOC) in influent wastewater (IWW) samples. Key mutations were targeted in the different VOC strains, including S Delta 69/70 deletion, N501Y, S Delta 241 and S Delta 157. T... Mehr ...
Verfasser: | |
---|---|
Dokumenttyp: | journalarticle |
Erscheinungsdatum: | 2022 |
Schlagwörter: | Biology and Life Sciences / wastewater-based epidemiology / variants of concern / digital polymerase / chain reaction / Belgium / SARS-CoV-2 |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-27369108 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://biblio.ugent.be/publication/8756155 |