Introduction Corn is an important cereal with a lot of applications in the feed and food industries (e.g. starch production). To evaluate properly the environmental impact of its applications, for example in the growing context of biobased products, a better understanding of the impact of its production is needed, using Life Cycle Analysis (LCA). Any error made during the agricultural step could have a large impact on subsequent results obtained at the final product stage. The objective of this study is to have a good understanding of environmental impact of corn production in Belgium context with a special focus on water depletion. Materials and methods The studied system is the production of corn in Wallonia (South of Belgium), whose the primary data are taken from Van Stappen et al [1]. The functional unit is 1 hectare of corn crop in Wallonia. The LCI data are based on actual agricultural practices recorded in farms’ accounting data. The field emissions due to the application of inputs were assessed by emission models as recommended by Nemecek [2] The system has been modelled in GaBi 8 using GaBi datasets. Belgian datasets have been preferred when available, and if not, European datasets are used and if no European datasets are available, then German ones have been used. The ILCD recommended method has been used for the impact evaluation. To have a better understanding of water depletion, some other methodologies have been tested for this specific impact categorie such as ReCiPe [3] and the AWARE model [4]. In ReCiPe, the water depletion is not regionalized whereas it is the case in the two other methods. The ReCipe method only adds the different water flow consumption to calculate the water depletion. The ILCD recommended method for water depletion is based on Swiss Ecoscarity model [5]. In this case, the water footpint is based on the water withdrawal-to-availability (WTA). This indicator measures the fraction of the water withdrawal, i.e., the total water input into a product system, which has become ...