Estudio histológico y morfológico del desarrollo embrionario del pez capitán de la sabana (Eremophilus mutisii) ; Histological and morphological study of the embrionary development of the capitan de la sabana (Eremophilus mutisii)

Eremophilus mutisii, due to its vulnerable condition and its potential for fish farming, generates research challenges; one of them is to know the embryonic changes of the E. mutisiibased on morphological and histological features from fer-tilization to hatching. The embryos were obtained from adult and sexually mature individuals of the Capitán de la Sabana (Eremophilus mutisii), sexually induced with carp pituitary extract. Oocytes and semen were obtained by abdominal pressure 24 hours after the last dose at 14±1°C. Sampling was performed every 60 minutes for the first twelve hours ́ post-fe... Mehr ...

Verfasser: Moncaleano Gómez, Erika
Sánchez, Carlos
Prieto Mojica, Camilo
Dokumenttyp: Artículo de revista
Erscheinungsdatum: 2018
Verlag/Hrsg.: Bogotá : Universidad de Ciencias Aplicadas y Ambientales
2018
Schlagwörter: Capitán de la sabana / Desarrollo embrionario / Embriogénesis / Siluriformes / Trichomycteridae
Sprache: Spanish
Permalink: https://search.fid-benelux.de/Record/base-27262985
Datenquelle: BASE; Originalkatalog
Powered By: BASE
Link(s) : https://revistas.udca.edu.co/index.php/ruadc/article/view/1073

Eremophilus mutisii, due to its vulnerable condition and its potential for fish farming, generates research challenges; one of them is to know the embryonic changes of the E. mutisiibased on morphological and histological features from fer-tilization to hatching. The embryos were obtained from adult and sexually mature individuals of the Capitán de la Sabana (Eremophilus mutisii), sexually induced with carp pituitary extract. Oocytes and semen were obtained by abdominal pressure 24 hours after the last dose at 14±1°C. Sampling was performed every 60 minutes for the first twelve hours ́ post-fertilization (HPF); from here on at hatching, the sam-ples were fixed every 6 hours. The embryos were fixed in 4% formalin for observing the morphological features and for the histological studies. Results revealed that the fertilized eggs had spherical shape, light green color and average diameter of 1270μm. The first cleavage at 2 HPF was observed, the blastulation began at 9 HPF. Epibolic movements and gastru-lation were observed from 12 HPF to 30 HPF with blastopore closure. At 60 HPF the yolk sac tail detachment was evident and at 66 HPF the first autonomous movements began. Finally, at 72 HPF, the hatching began, showing absence of pigmentation and evidencing a rudimentary digestive tract without oral and anal opening. It was observed the presence of abundant yolk sac with continuous vertical movements of the larva. ; Eremophilus mutisii, pez catalogado en estado como vulnerable, que por su importancia sociocultural y ecológica genera retos investigativos, uno de ellos, conocer sus características histológicas del desarrollo embrionario, desde la fecundación hasta la eclosión. Para ello, se obtuvieron embriones de individuos adultos y maduros sexualmente, capturados en la represa del Sisga, ubicada en el municipio de Chocontá, Cundinamarca, inducidos sexualmente con extracto pituitario de carpa. Los ovocitos y el semen fueron obtenidos por presión abdominal 24 horas después de la última dosis, a 14±1°C. La toma de ...