Molecular diversity of Bacillus anthracis in the Netherlands: Investigating the relationship to the worldwide population using whole-genome SNP discovery
International audience ; Bacillus anthracis, the causative agent of anthrax, has been widely described as a clonal species. Here we report the use of both canonical SNP analysis and whole-genome sequencing to characterize the phylogenetic lineages of B. anthracis from the Netherlands. Eleven strains isolated over a 25-years period (1968-1993) were paired-end sequenced using parallel sequencing technology. Five canSNP groups or lineages, i.e. A.Br.001/002 (n = 6), A.Br.Aust94 (n = 2), A.Br.008/011 (n = 1), A.Br.011/009 (n = 1) and A.Br.Vollum (n = 1) were identified. Comparative analyses, with... Mehr ...
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Dokumenttyp: | Artikel |
Erscheinungsdatum: | 2015 |
Verlag/Hrsg.: |
HAL CCSD
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Schlagwörter: | [SDV]Life Sciences [q-bio] |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-27238867 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://anses.hal.science/anses-03857968 |
International audience ; Bacillus anthracis, the causative agent of anthrax, has been widely described as a clonal species. Here we report the use of both canonical SNP analysis and whole-genome sequencing to characterize the phylogenetic lineages of B. anthracis from the Netherlands. Eleven strains isolated over a 25-years period (1968-1993) were paired-end sequenced using parallel sequencing technology. Five canSNP groups or lineages, i.e. A.Br.001/002 (n = 6), A.Br.Aust94 (n = 2), A.Br.008/011 (n = 1), A.Br.011/009 (n = 1) and A.Br.Vollum (n = 1) were identified. Comparative analyses, with a focus on SNPs discovery, were carried out using a total of 52 B. anthracis genomes. A phylogeographic ''Dutch'' cluster within the dominant A.Br.001/002 group was discovered, involving isolates from a single outbreak. Diagnostic SNPs specific to the newly identified subgroups were developed into high-resolution melting SNP discriminative assays for the purpose of rapid molecular epidemiology. Phylogenetic relationships with strains from other parts of the world are discussed.