The Drentsche Aa valley system in the glacial sandy landscape of the north-eastern Netherlands, is one of the many regional drainage systems in the north-western to central European Lowlands. Following deep incision in the Weichselian, ca 7 m of eutrophic peat has accumulated in the lower to middle reaches of the valley system in the Holocene, completely filling the Weichselian incision. We reconstructed the rate of water-level rise controlling peat growth at three locations in the valley system (representing the upper, middle and lower reaches), using 14C peat dates from compaction-free sampling sites on the sloping valley margin where the peat directly overlies the sandy Pleistocene subsurface. The 14C dates enabled the construction of curves showing the water-level evolution at each location. Our results show variable rates of Holocene peat growth in the valley system in response to different drivers of hydrological change: a strong increase in annual precipitation (onset Holocene), a decrease in evapotranspiration (10,000–8000 cal. years BP) and relative sea-level rise (after ~4500 cal. years BP). Because the identified hydrological controls on peat growth are regional, they probably ruled Holocene peat growth in many peat-filled valley systems in the north-western to central European Lowlands. Currently, there is growing attention for these valley systems in the context of European nature restoration efforts and climate change adaptation and mitigation measures. The present study underscores that large-scale hydrological measures outside the valley system are needed for restoration of degraded valley peatlands.