Sequence and functional characterization of RNase P RNA from the chl a/b containing cyanobacterium Prochlorothrix hollandica
Only a few complete sequences and very limited functional data are available for the catalytic RNA component of cyanobacterial RNase P. The RNase P RNA from the chl a / b containing cyanobacterium Prochlorothrix hollandica belongs to a rarely found structural subtype with an extended P15/16 domain. We have established conditions for optimal in vitro ribozyme activity, and determined the kinetic parameters for cleavage of pre‐tRNA Tyr . Analysis of pre‐tRNA mutants revealed that the T‐stem sequence only plays a modulating role, whereas the CCA end is essential for efficient product formation.... Mehr ...
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| Dokumenttyp: | Artikel |
| Erscheinungsdatum: | 1998 |
| Reihe/Periodikum: | FEBS Letters ; volume 428, issue 3, page 161-164 ; ISSN 0014-5793 1873-3468 |
| Verlag/Hrsg.: |
Wiley
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| Schlagwörter: | Cell Biology / Genetics / Molecular Biology / Biochemistry / Structural Biology / Biophysics |
| Sprache: | Englisch |
| Permalink: | https://search.fid-benelux.de/Record/base-27122201 |
| Datenquelle: | BASE; Originalkatalog |
| Powered By: | BASE |
| Link(s) : | http://dx.doi.org/10.1016/s0014-5793(98)00519-5 |
Only a few complete sequences and very limited functional data are available for the catalytic RNA component of cyanobacterial RNase P. The RNase P RNA from the chl a / b containing cyanobacterium Prochlorothrix hollandica belongs to a rarely found structural subtype with an extended P15/16 domain. We have established conditions for optimal in vitro ribozyme activity, and determined the kinetic parameters for cleavage of pre‐tRNA Tyr . Analysis of pre‐tRNA mutants revealed that the T‐stem sequence only plays a modulating role, whereas the CCA end is essential for efficient product formation.