This article contributes to the debate about the role of affect in transformative change towards gender equality, by comparing the building of affect in two recently founded women’s networks in Italian and Dutch universities. By conceptualizing networking as a social and cultural practice that organizes a collective body through the building of affect between specific groups of organizational stakeholders, we reveal the emotional, dynamic and context-dependent character of transformative change. We found that similar women’s networks build affect with organizational stakeholders in different ways, shaping boundaries of different collective bodies through emotions and adapting to the cultural meaning attributed to earlier actions. By bridging bottom-up and top-down approaches to gender equality and tailoring the building of affect to the local context they potentially contribute to transformational change towards gender equality.