Expression in Escherichia coli and sequencing of the coding region for the capsid protein of Dutch maedi-visna virus strain ZZV 1050: application of recombinant protein in enzyme-linked immunosorbent assay for the detection of caprine and ovine lentiviruses
Maedi-visna in sheep and caprine arthritis-encephalitis in goats are caused by two closely related and widespread lentiviruses. The infections are characterized by life-long virus persistence and slow induction of antiviral antibodies. The diagnosis is based on the detection of antiviral antibodies. We have used the polymerase chain reaction (PCR) to amplify a part of the gag gene coding for the entire capsid protein and for parts of the matrix and nucleocapsid proteins. Sequencing of the PCR fragment of the Dutch maedi-visna virus strain ZZV 1050 revealed 85 and 92% homology to the DNA and de... Mehr ...
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Dokumenttyp: | Artikel |
Erscheinungsdatum: | 1991 |
Reihe/Periodikum: | Journal of Clinical Microbiology ; volume 29, issue 7, page 1290-1294 ; ISSN 0095-1137 1098-660X |
Verlag/Hrsg.: |
American Society for Microbiology
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Schlagwörter: | Microbiology (medical) |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-27031812 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | http://dx.doi.org/10.1128/jcm.29.7.1290-1294.1991 |