Cryopreservation and Micropropagation Methods for Conservation of Genetic Resources of Ulmus laevis and Ulmus glabra

Elms are threatened by Dutch elm disease, and conservation methods are needed to protect their genetic diversity. Cryopreservation of dormant buds allows large numbers of genotypes to be conserved with small space requirements and minimal upkeep. Cryopreservation through slow controlled cooling was tested for both elm species native to Finland, Ulmus glabra and Ulmus laevis . Regeneration of the thawed buds by micropropagation was studied on different basal media and using different growth regulators. Multiple surface sterilisation methods were tried out for bud explants. The multiplication of... Mehr ...

Verfasser: Sakari Välimäki
Mari Rusanen
Daniela Pečínková
Mikko Tikkinen
Tuija Aronen
Dokumenttyp: Artikel
Erscheinungsdatum: 2021
Reihe/Periodikum: Forests, Vol 12, Iss 1121, p 1121 (2021)
Verlag/Hrsg.: MDPI AG
Schlagwörter: wych elm / European white elm / Dutch elm disease / vegetative propagation / tissue culture / Plant ecology / QK900-989
Sprache: Englisch
Permalink: https://search.fid-benelux.de/Record/base-27020495
Datenquelle: BASE; Originalkatalog
Powered By: BASE
Link(s) : https://doi.org/10.3390/f12081121

Elms are threatened by Dutch elm disease, and conservation methods are needed to protect their genetic diversity. Cryopreservation of dormant buds allows large numbers of genotypes to be conserved with small space requirements and minimal upkeep. Cryopreservation through slow controlled cooling was tested for both elm species native to Finland, Ulmus glabra and Ulmus laevis . Regeneration of the thawed buds by micropropagation was studied on different basal media and using different growth regulators. Multiple surface sterilisation methods were tried out for bud explants. The multiplication of U. glabra was investigated with Driver and Kuniyuki walnut medium with either 0.5 mg/L meta-topolin or 0.5 mg/L 6-benzylaminopurine. Rooting with short indole-6-butyric acid induction in liquid medium and direct transplantation of the shoots to peat ex vitro after induction were tested. For initiation, either Murashige and Skoog or Driver and Kuniyuki walnut medium with 0.02 mg/L gibberellic acid 4 + 7 and 0.5 mg/L 6-benzylaminopurine were found to best promote shoot formation. Surface sterilisation remains the most challenging step. No significant differences were found between the multiplication media in either shoot production or rooting success. Rooting by direct transplanting was achieved in both species, but further development is required before application on a larger scale. With further improvements to sterilisation success especially in U. glabra , the method can be applied to the conservation of genetic resources of both U. laevis and U. glabra , and knowledge of regeneration success can be used to design the cryoconservation plan and optimise the sampling.