Potential Dutch elm disease vector beetle species were caught with pheromone bottle traps and handpicked in 2019: in total, seven species and 261 specimens were collected. The most common was Scolytus triarmatus , but by percent, the incidence of Ophiostoma novo-ulmi was highest in Scolytus scolytus , followed by Xyleborinus saxesenii and S. triarmatus . We analysed the beetles ’ DNA using PacBio sequencing to determine vector beetles of Ophiostoma novo-ulmi . Ophiostoma novo-ulmi was found on six out of seven analysed beetle species: Scolytus scolytus , S . triarmatus , S. multistriatus , S. laevis , Xyleborinus saxesenii and Xyleborus dispar . The last two beetles were detected as vectors for Ophiostoma novo-ulmi for the first time. Previous knowledge on the spread of beetles is discussed.