Abstract Background Physical activity (PA) reduces 19.5% of the attributable burden of chronic diseases (CDs), i.e. healthy life years lost due to morbidity and mortality. In addition, 29.5% of Belgians self-declared having at least one chronic disease. Moreover, almost 30% of the population above 15 years old is at health risk due to physical inactivity. Evidence shows physical activity on prescription (PAP) has a positive impact on PA level and metabolic health. General Practitioners (GPs) can use PAP as a prevention intervention. In Belgium, overall 94,1% of citizens have a referring General Practitioner (GP) or are affiliated with a general practice. There is no national policy on PAP, only a regional policy in Flanders. Therefore, the aim of the study is to investigate the perception and the (potential) provision of PAP by Belgian GPs. Methods A cross-sectional survey was conducted in Belgian GPs between January and end of March 2023. The survey has 41 (ordinal or multiple choice) questions. Descriptive statistics were performed. Results 134 GPs completed the survey of which 56% were female. 66.4% work in Flanders, 19.4% in Wallonia and 14.2% in Brussels-Capital region. Among respondents, 44% only discuss the benefits of PA with patients with a CD. Interestingly, less than 2% never discuss PA. 86.8% (would) prescribe if patients’ health status would be remedied by increasing PA, while 35.8% (would) use PAP if patients are inactive, regardless of their health status. About 75% identified chronic disease and exercise initiation as incentives for PAP. 41% would refer to PA (motivational) coaches, 55.2% to (adapted) PA courses. Lack of training (12.7%), time (49.3%), and knowledge of local PA offer (74%) were cited as barriers to using PAP. Conclusions The majority of responding GPs linked PAP with CD. Although there is no national implementation and regulation of PAP, GPs used (were ready to use) PAP in their practice. However, several barriers still hinder the use of PAP. Key messages • PAP is identified by ...