Optimization and Application of a Multiplex Digital PCR Assay for the Detection of SARS-CoV-2 Variants of Concern in Belgian Influent Wastewater
Since the beginning of the COVID-19 pandemic, the wastewater-based epidemiology (WBE) of SARS-CoV-2 has been used as a complementary indicator to follow up on the trends in the COVID-19 spread in Belgium and in many other countries. To further develop the use of WBE, a multiplex digital polymerase chain reaction (dPCR) assay was optimized, validated and applied for the measurement of emerging SARS-CoV-2 variants of concern (VOC) in influent wastewater (IWW) samples. Key mutations were targeted in the different VOC strains, including SΔ69/70 deletion, N501Y, SΔ241 and SΔ157. The presented bioan... Mehr ...
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Dokumenttyp: | Text |
Erscheinungsdatum: | 2022 |
Verlag/Hrsg.: |
Multidisciplinary Digital Publishing Institute
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Schlagwörter: | wastewater-based epidemiology / variants of concern / digital polymerase chain reaction / Belgium / SARS-CoV-2 |
Sprache: | Englisch |
Permalink: | https://search.fid-benelux.de/Record/base-26925731 |
Datenquelle: | BASE; Originalkatalog |
Powered By: | BASE |
Link(s) : | https://doi.org/10.3390/v14030610 |