Digestive aspartic proteases from sábalo (Prochilodus lineatus): Characterization and application for collagen extraction
Acid proteases from sábalo stomach mucosa were recovered using salting-out procedure. This single step produced an enzyme extract purified 1.8-fold over the crude extract with a recovery of 45.1% of its initial proteolytic activity. Sábalo proteases exhibited the highest activity at 45 °C-pH 2.0, showed pH stability between 2.0 and 5.0 and retained more than 70% of its activity after incubation at pH 7.0 for 2 h. Fish extract was unstable at temperatures greater than 45 °C. Its activity was inhibited by pepstatin A but not by PMSF, while EDTA and SDS showed partial inhibitory effects. Presence... Mehr ...
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| Dokumenttyp: | Artikel |
| Verlag/Hrsg.: |
Elsevier
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| Schlagwörter: | ACETIC ACID (PUBCHEM CID: 176) / ACIDIC PROTEASE / BUTYL ALCOHOL (PUBCHEM CID: 263) / COLLAGEN / ETHYLENEDIAMINETETRAACETIC ACID (PUBCHEM CID: 6049) / GLYCINE (PUBCHEM CID: 750) / HYDROCHLORIC ACID (PUBCHEM CID: 313) / METHANOL (PUBCHEM CID: 887) / PHENYLMETHYLSULFONYL FLUORIDE (PUBCHEM CID: 4784) / SODIUM CHLORIDE (PUBCHEM CID: 5234) / SODIUM DODECYL SULFATE (PUBCHEM CID: 3423265) / SODIUM HYDROXIDE (PUBCHEM CID: 14798) / STOMACH / SÁBALO (PROCHILODUS LINEATUS) / https://purl.org/becyt/ford/1.4 / https://purl.org/becyt/ford/1 |
| Sprache: | Englisch |
| Permalink: | https://search.fid-benelux.de/Record/base-26889484 |
| Datenquelle: | BASE; Originalkatalog |
| Powered By: | BASE |
| Link(s) : | http://hdl.handle.net/11336/96187 |
Acid proteases from sábalo stomach mucosa were recovered using salting-out procedure. This single step produced an enzyme extract purified 1.8-fold over the crude extract with a recovery of 45.1% of its initial proteolytic activity. Sábalo proteases exhibited the highest activity at 45 °C-pH 2.0, showed pH stability between 2.0 and 5.0 and retained more than 70% of its activity after incubation at pH 7.0 for 2 h. Fish extract was unstable at temperatures greater than 45 °C. Its activity was inhibited by pepstatin A but not by PMSF, while EDTA and SDS showed partial inhibitory effects. Presence of CaCl 2 and MgCl 2 increased the proteolytic activity, while increasing concentrations of NaCl strongly decreased it. In addition, compared to the acid extraction method, the use of sábalo enzymatic extract increased 1.7 times the yield of collagen extraction. ; Fil: Acevedo Gomez, Antonella Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina ; Fil: Gomez, Gabriela Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina ; Fil: Chamorro, Ester Ramona. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina ; Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y ...