CARACTERIZACIÓN MOLECULAR PARCIAL DEL VIRUS DEL MOTEADO DEL CLAVEL (CarMV) PRESENTE EN LA SABANA DE BOGOTÁ
Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SDS-PAGE showing a single component of about 38 Kda. Double-stranded RNA (dsRNA) extracted from carnation infected tissue showed 3 to 5 electrophoretic components ranging between 8.0 and 0.9 Kbp. Virus particles contained a genomic RNA of about 4.0 Kbp as revealed by agarosa gel electrophoresis. It wa... Mehr ...
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| Dokumenttyp: | http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| Erscheinungsdatum: | 2018 |
| Verlag/Hrsg.: |
Pontificia Universidad Javeriana
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| Schlagwörter: | null / cápside viral / carmovirus / clavel / dsRNA / electroforesis |
| Sprache: | Englisch |
| Permalink: | https://search.fid-benelux.de/Record/base-26879736 |
| Datenquelle: | BASE; Originalkatalog |
| Powered By: | BASE |
| Link(s) : | http://hdl.handle.net/10554/31014 |
Using an ELISA test, camation mottle virus (CarMV) was found to infect 82% of the camation samples collected from the Sabana of Bogotá. The isolated virus was mechanically inoculated on virus-free carnation plants and purified by sucrose gradient centrifugation. CarMV coat protein was analized by SDS-PAGE showing a single component of about 38 Kda. Double-stranded RNA (dsRNA) extracted from carnation infected tissue showed 3 to 5 electrophoretic components ranging between 8.0 and 0.9 Kbp. Virus particles contained a genomic RNA of about 4.0 Kbp as revealed by agarosa gel electrophoresis. It was posible to distinguished 2 groups of electrophoretic pattems using RNAse T1 digestion of the RNA contained in the CarMV purified particles.