Contribución a la relación taxonómica entre cuatro especies de peces de la familia Characidae mediante el Polimorfismo de ADN Amplificado al Azar (RAPD) ; Contribution taxonomic relationship of the among four fish species of the family Characidae by the use of Random Amplified Polymorphic DNA (RAPD)
ABSTRACT: Information on taxonomic relationship among fish species is fundamental for an identification of wild populations. The family Characidae is a fish group widely distributed in Central and South America which must be identified, classified and preserved. Random Amplified Polymorphic DNA (RAPD) in this was used study to evaluate the taxonomic relationship in four tropical freshwater fish species located in Colombian territory, which belong to the family Characidae; subfamily Bryconinae: Brycon moorei and Brycon henni; and subfamily Serrasalminae: Colossoma macropomum and Piaractus brach... Mehr ...
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| Dokumenttyp: | Artikel |
| Erscheinungsdatum: | 2004 |
| Verlag/Hrsg.: |
Universidad de Antioquia
Facultad de Ciencias Agrarias |
| Schlagwörter: | RAPD (DNA polimorfismo amplificado aleatorio) / Sabaleta : Brycon henni / Brycon (Pisces : Characidae) / Dorada : Brycon moorei / Cachama negra : Colossoma macroponum / Cachama blanca : Piaractus brachypomus |
| Sprache: | Spanish |
| Permalink: | https://search.fid-benelux.de/Record/base-26875790 |
| Datenquelle: | BASE; Originalkatalog |
| Powered By: | BASE |
| Link(s) : | http://hdl.handle.net/10495/7286 |
ABSTRACT: Information on taxonomic relationship among fish species is fundamental for an identification of wild populations. The family Characidae is a fish group widely distributed in Central and South America which must be identified, classified and preserved. Random Amplified Polymorphic DNA (RAPD) in this was used study to evaluate the taxonomic relationship in four tropical freshwater fish species located in Colombian territory, which belong to the family Characidae; subfamily Bryconinae: Brycon moorei and Brycon henni; and subfamily Serrasalminae: Colossoma macropomum and Piaractus brachypomus. Thirty four out of forty primers with sequences of ten nucleotides at random (RAPD) yielded 2.129 amplified DNA fragments, 428 of them were identified as specific markers that discriminated between genus and species. The fragment profiles showed that Brycon moorei got apart from the Brycon henni group, and inside this last group was possible to discriminate between samples from two different riverbasins; Magdalena and Cauca rivers. Piaractus brachypomus and Colossoma macropomum got apart from each other and distant from the subfamily Bryconinae. Furthermore, inside the Piaractus brachypomus group was possible to discriminate among individuals from the wild or the culture. The RAPD technique allowed an approach to the systematics in these species toward an appropriate identification. ; RESUMEN: La información sobre la relación taxonómica entre especies de peces es fundamental para una identificación de las poblaciones naturales. La familia Characidae es un grupo de peces ampliamente distribuido en Centro y Suramérica el cual debe ser identificado, clasificado y preservado. En este estudio fue utilizado el Polimorfismo de ADN Amplificado al Azar (RAPD) para evaluar la relación taxonómica en cuatro especies tropicales de peces continentales localizados en el territorio colombiano, los cuales pertenecen a la familia Characidae; subfamilia Bryconinae: Brycon moorei y Brycon henni; y subfamilia Serrasalminae: Colossoma ...