Currently, there is a high dependence in border areas of Colombia for the constant supply of petroleum fuels. The biofuels generation from low-cost biological fat materials, easy acquisition and minimizing environmental impacts, are research topics. The objective of this work, was to evaluate the physicochemical characteristics of biodiesel derived from chicken fat, which is managed as agroindustrial waste of the transformation process in the poultry farms of the Sabana Sub Region, Nariño Department, Colombia. Chicken fat oil was evaluated to extraction, purification and transesterification processes by means of basic catalysis, using sodium hydroxide in an alcoholic phase of methanol. The results of the chicken fat biodiesel analysis comply with the stipulations of NTC 5444, with the exception of the viscosity parameter (7.5 mm2/s at 40 ºC), which is higher than the one established in the rule. Chicken fat is an alternative raw material in the production of conventional biodiesel in the same production areas, which meets the specifications of Colombian regulations according to NTC 5444, and could be used in any diesel combustion engine, with benefits like a lower sulfur content and contributing to the research for sustainability. ; Actualmente, existe una alta dependencia en zonas limítrofes de Colombia por el suministro constante de combustibles derivados del petróleo. La generación de biocombustibles a partir de materiales grasos biológicos de bajo costo, fácil adquisición y que minimicen los impactos ambientales, son temas de investigación. El objetivo de este trabajo fue evaluar las características fisicoquímicas de biodiésel derivado de grasa de pollo, la cual es manejada como residuo agroindustrial del proceso de trasformación en las granjas avícolas de la subregión de la sabana de Túquerres del departamento de Nariño, Colombia. Se evaluó la grasa de pollo ó aceite de pollo sometido a procesos de extracción, purificación y transesterificación, por medio de una catálisis básica, utilizando como catalizador ...